The Cellufine™ ET clean is poly(ε-lysine) immobilized Cellufine™ (cellulose spherical beads). The beads bind and remove endotoxin from your sample solution. The poly(ε-lysine) is a microbial poly(amino acid) that consist of 25-35 lysine residues produced by Streptomyces albulus. The poly(ε-lysine) as ligand and the cellulose beads act as matrix ands are products of JNC Corporation.
The Cellufine™ ET clean endotoxin removing beads were developed jointly by Kumamoto University and Chisso. The poly(ε-lysine) was immobilized onto chloromethyloxirane-activated cellulose beads. The beads are a stable affinity beads that are resistant against the cleanup solutions, which include 0.2 M sodium hydroxide and 2 M sodium
The Cellufine™ ET clean can remove endotoxin from a cellular product solution at physiological pH, ionic strength of μ = 0.02-1.0, and 0° -25C°.
|Name||Supplied||Wet Bead Diameter||Pore Size*|
|Cellufine™ ET clean S||a slurry in 20 % ethanol||ca. 40-130 μm||Mlim 2000|
|Cellufine™ ET clean L||a slurry in 20 % ethanol||ca. 40-130 μm||>Mlim 2x106|
*The pore size (molecular weight exclution; Mlim) of the beads was estimated from calibration curves obtained by size exclusion chromatography. Pullulan and maltose were used for the Mlim determination.
Selective adsorption of endotoxin (LPS) from a bovine serum albumin (BSA) solution by Cellufine ET clean beads.
Selective adsorption of endotoxin was determined using a batchwise method with 0.2 g of the wet beads and 2 ml of a sample solution (BSA: 500 μg/ml, E. coli O111: B4 LPS: 100 ng/ml, pH 7.0, ionic strength of μ = 0.05-0.8 ).
Selective removal of endotoxin from a protein solution by Cellufine™ ET clean beads.
|Sample Solution||Cellufine™ ET clean S||Cellufine™ ET clean L|
|Compound(pI)||Concentration of endotoxin before treatment(pg/ml)||(μ = 0.05, pH 7.0)||(μ = 0.4, pH 7.0)|
|Concentration of endotoxin after treatment(pg/ml)||Recovery of protein after treatment(%)||Concentration of endotoxin after treatment(pg/ml)||Recovery of protein after treatment(%)|
The removal of endotoxin was determined by a batchwise method with 0.3 ml of wet adsorbent and 2 ml of a protein solution (1 mg/ml) containing natural endotoxin.
- Column size
- 1 X 1.1 cm (I.D.) (1.1ml)
- Flow rate
- 0.17 ml / min (10cm / h)
- 50 mM PB, pH 7 + 0.15 mol NaCl aq
- Column size
- 10 x 0.9 cm (I.D.) (9.6 ml)
- Flow rate
- 0.5 ml / min (47 cm / h)
- 1 mM Tris-HCl, pH 7.3
- 0 → 1.0 mol / l NaCl aq.
1) M. Sakata, M. Todokoro, C. Hirayama, American Biotechnol. Lab., 20 (2002) 36.
2) M. Todokoro, M. Sakata, S. Matama, M. Kunitake, J. Ohkuma, C. Hirayama, J. Liq. Chrom. & Rel. Technol., 25 (2002) 601.