Cellufine Resin Application List

At this location on the JNC web site are a range of Application Technical Notes describing recent purification of and polishing of monoclonal antibodies and whole viral vaccine particles. These technical articles are useful as a resource for development of your downstream process workflow with Cellufine products.

Purification of mAb from CHO culture supernatant with Cellufine products: Three step purification workflow

mAb’s derived from CHO cell culture supernatant can be purified with a conventional three step chromatography workflow. Initial mAb capture with Cellufine SPA-HC is applied followed by polishing with Cellufine MAX Q-h and Cellufine MAX GS.

Efficient mAb purification process using Cellufine chromatography media: Direct coupling of two polishing workflow steps (FT-FT mode)

In most downstream monoclonal antibody (mAb) purification processes, initial Protein A capture is usually followed by two polishing steps such as AEX followed by CEX. In this report, we have demonstrated an improved polishing workflow by directly connecting two flow-through chromatography steps (FT-FT mode): Cellufine MAX IB mixed mode and Cellufine GS cation exchange.

Two step purification of mAb with Cellufine rProA Affinity and Mixed Mode Chromatography Resins

In Technical Note, we introduce a two steps purification method using rProtein A chromatography for mAb capture and Cellufine MAX IB mixed mode as a second step polishing process. By efficiently combining capture and polishing it is possible to purify a mAb in a cost-effective workflow with minimal time spent moving between the two chromatography modes.

Purification of Human Coronavirus hOC43 with Cellufine Sulfate

Cellufine Sulfate has been widely used in virus purification. βcoronavirus hCoV OC43 has been purified with Cellufine Sulfate and is described in this Technical Note. This pseudo affinity chromatography resin has proved to be highly effective in the purification of βcoronavirus.

Two-step purification of T7 RNA Polymerase with Cellufine MAX DEAE and Phosphate Affinity Chromatography Resins

T7 RNA polymerase is an enzyme derived from T7 phage used to produce mRNA transcripts from the template DNA. In the manufacture of COVID-19 mRNA vaccines. In this report, we will introduce an example of purification of T7 RNA polymerase. T7 RNA polymerase was expressed in Escherichia coli (pAR1219) and then purifying the fermented solution with Cellufine Phosphate and Cellufine MAX DEAE (weak anion exchanger) to high purity. High-purity T7 RNA polymerase was able to be purified with Cellufine Phosphate, which is an affinity chromatography resin.

Purification of Capsular Polysaccharides of Streptococcus pneumoniae Serotype 19F by Cellufine

Streptococcus pneumoniae is one of the major pathogens causing high level morbidity and mortality worldwide, especially children and the elderly populations. Pneumococcal vaccines based on the capsular polysaccharide (CPS) on the bacteria surface, which is one of the most important virulence factors, have been used to prevent these infectious diseases. Traditional ethanol precipitations are general processes for purifying CPSs, which are suitable for most serotypes. However, they are complex and time consuming. As a result, pneumococcal vaccines are quite expensive. Here we would like to propose simplified two step chromatography purification process of CPS without ethanol precipitations. This process includes hydrophobic interaction chromatography (HIC) and anion exchange chromatography (AEX). We introduce two optimized resins, Cellufine MAX Butyl HS (HIC) and Cellufine MAX Q-hv (AEX), for this process.

Custom Resin Development Services

JNC offers a custom resin development service for optimization of current products to meet stringent or enhanced performance working directly with large and small customers. In addition, JNC also offers custom resin development services to support new applications of JNC resins.

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