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Introduction of developed products：
Cellulose resin MLP1000 DexS

What is Cellulose resin MLP1000 DexS?

The cellulose resin MLP1000 is a highly crosslinked cellulose particle that has a large continuous pore structure. In the purification of large virus and virus-like particles, the intraparticle surface of the resin can be used as an adsorption site, so the MLP1000 DexS exhibits high binding capacity and purification capabilities.

How to request products under development

The Cellulose resin MLP1000 DexS is an under developing product. The quality of the Cellufine which are chromatography media are guaranteed by ISO 9001, but the quality of this product is subject to change as it is under development.
This product is not sold. We provide a small amount of samples as a developing product. For pharmaceutical companies, diagnostics companies or other manufacturing companies and researchers who would like to handle this product for manufacturing process, we will provide samples those who consenting to above fact.
Please contact the following for sample requests.

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Cellulose resin MLP1000 DexS

The cellulose resin MLP1000 is a highly crosslinked cellulose particle that has a large continuous pore structure. In the purification of large virus and virus-like particles, the intraparticle surface of the resin can be used as an adsorption site, so the MLP1000 DexS exhibits high binding capacity and purification capabilities.

MLP1000 SEM
Fig.1 SEM photography of MLP1000
MLP1000 pore size
Fig.2 Retention times of PEG and PEO in MLP1000
After packing the resin into 7.8 mm ID x 300 mm column, polyethylene glycol (PEG) or polyethylene oxide (PEO) with different index molecular weights was passed through the column at a flow rate of 0.4 mL/min to confirm the retention time.

Table 1 shows the characteristics of the cellulose resin MLP1000 DexS.

Base resin Cross linked cellulose
Particle size Ave.90μm (40 – 150 μm)
Ligand Dextran sulfate
Pressure < 0.3MPa

Table 1 Characteristics of MLP1000 DexS

The cellulose resin MLP1000 DexS is a highly crosslinked cellulose particle. Despite having extremely large open pores for virus particles to enter, the resin has high pressure resistance and can be used in large columns for the production of biopharmaceuticals.

Pressure flow curve of Cellulose resin MLP1000 DexS
Fig.3 Flow characteristics of MLP1000 DexS
After packing the resin into a 2.2 cm ID x 20 cm column, pure water at 24°C ± 1°C was used as the mobile phase, and the relationship between flow rate and pressure drop was evaluated.

Example of Purification of Inactivated Influenza Virus

Chromatography purification was performed using allantoic fluid containing influenza virus H1N1.

purification for ful with mlp
Fig.4 Dynamic binding capacity of Inactivated Influenza Virus
It shows the dynamic bindigng capacity of influenza virus. Chromatography conditions are as follows.

Column: I.D. 5.0 x 15 mm (0.3 mL)
Load sample: Allantoic fluid from chicken egg containing inactivated influenza virus
Virus strain: A/Hyogo/YS/2011(H1N1)
Flow rate：0.5 ml/min(150 cm/h、R.T. 0.6 min)
Equilibration: 10 mM Na phosphate, 120 mM NaCl, pH 7.4
Wash: 10 mM Na phosphate, 120 mM NaCl, pH 7.4
Elution: 10 mM Na phosphate, 2 M NaCl, pH 7.4
Process Volume [mL] Virus activity [HA Value] Proteins [µg] dsDNA [µg] Recovery %
Allantoic fluid 20 99,600 10,100 6.4 100
Flow through 20 400 9,220 3.6 1
Wash 6 1,560 786 0.3 2
Elution 1.5 92,880 975 1.4 93

Table 2 Purification results of inactivated influenza virus

With chromatography using the cellulose resin MLP1000 DexS, the liquid volume was reduced from 20 mL to 1.5 mL and the virus could be concentrated up to 13-fold. The amount of protein impurities was reduced from 10,100 µg to 975 µg, resulting in a 90.3% reduction. It was also able to reduce the amount of dsDNA to 22% as well.

Example of Purification of Human coronavirus OC43

Human coronavirus OC43 (hCoV OC43) is a common cold-causing virus of approximately 100 nm in size belonging to the same beta coronavirus genus as SARS-CoV-2 that causes COVID-19 infection. We present an example of the purification of human coronavirus OC43 cultured in Vero cells by chromatography.

purification for ful with mlp
Fig.4 Dynamic binding capacity of Inactivated Human coronavirus OC43
It shows the dynamic bindigng capacity of Human coronavirus OC43. Chromatography conditions are as follows.

Column: I.D. 5.0 x 15 mm (0.3 mL)
Load sample: Cell culture supernatant fluid containing inactivated hCoV OC43
Flow rate：0.3 ml/min(90 cm/h、R.T. 1.0 min)
Equilibration: 10 mM Na phosphate, 150 mM NaCl, pH 7.4
Wash: 10 mM Na phosphate, 150 mM NaCl, pH 7.4
Elution: 10 mM Na phosphate, 2 M NaCl, pH 7.4
Elution 1：gradient (0→25%), 25 CV
Elution 2：isocratic (100%), 10 CV
Process Volume [mL] Virus activity [HA Value] Proteins [µg] dsDNA [µg] Recovery %
Supernatant fluid 34.5 120,186 6,394 52.79 100
Flow through 34.5 1,020 4,376 55.53 0.8
Wash 4.5 90 57 2.25 0.1
Elution (gradient, isocratic) 10.5 108,990 170 4.95 91

Table 3 Purification results of inactivated human coronavirus OC43

With the chromatographic purification using the cellulose resin MLP1000 DexS, the amount of protein impurities was reduced from 6394 µg to 170 µg, resulting in a 97.3% reduction. It was also able to reduce the amount of dsDNA to 9% in the viral elution fraction, achieving a final virus recovery rate of 91%, which showed a high recovery performance.

Custom Resin Development Services

JNC offers a custom resin development service for optimization of current products to meet stringent or enhanced performance working directly with large and small customers. In addition, JNC also offers custom resin development services to support new applications of JNC resins.

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