APPLICATION
Application example of ε-Polylysine
PROCESSING AID
Removal and recovery of DNA with ε-Polylysine.
Removal and recovery of DNA with ε-polylysine.Due to the interaction between the cation site of ε-Polylysine and the anion site of bacterial cell, etc., it exhibits strong agglutinating properties. Using this phenomenon, it is also possible to remove and recover DNA in solution by aggregating and precipitating DNA.
The action of ε-Polylysine on DNA derived from salmon semen.
Crude purification of fermentation broth with ε-Polylysine
By using ε-Polylysine for the crude purification of fermentation broth of lactase-producing bacteria (K. lactis), it was possible to obtain a crude enzyme solution in which the activity of the contaminant protease was reduced.
| Samples | A280 | Lactase (U/mL) |
Protease (Try, ppm) |
|---|---|---|---|
| Contorol | 1.91 | 1.72 | 191 |
| ε-Polylysine 40 ppm | 2.02 | 1.76 | 221 |
| ε-Polylysine 100 ppm | 2.21 | 1.75 | 242 |
| ε-Polylysine 400 ppm | 0.33 | 1.72 | 5 |
| ε-Polylysine 700 ppm | 0.38 | 1.71 | 4 |
| ε-Polylysine 1,000 ppm | 0.39 | 0.54 | 0 |
