Hydrophobic Interaction
Chromatography Media

CellufinecTM MAX Butyl
CellufineTM MAX Phenyl
CellufineTM MAX Phenyl LS

CellufineTM MAX is a 2nd generation Cellufine™ media with high flow characteristics. JNC developed a new, highly cross-linked base resin for Cellufine™ MAX series. Cellufine™ MAX hydrophobic interaction chromatography is now available with MAX Phenyl and Butyl chemistries.

Cellulose, a natural polysaccharide, possesses unique crystalline molecular structure differing from non-crystalline polysaccharides such as agarose. Thus Cellufine™ has distinctive pore structure as shown in the pictograph (Fig. 1). The new Cellufine™ MAX series offers the largest pore size of all Cellufine™ chromatography media. The benefit of such pore size in Cellufine™ MAX HIC media provides superior strength and excellent mass transfer.

Partial structure

  • Hydrophobic Interaction

    Cellufine™ MAX Butyl

    Ligand structure of Cellufine™ MAX Butyl, phenyl group immobilized on spherical porous cellulose particles

  • Cellufine™ MAX Phenyl
    Cellufine™ MAX Phenyl LS

    Ligand structure of Cellufine™ MAX phenyl, phenyl group immobilized on spherical porous cellulose particles

Characteristics

Characteristics
Type Cellufine™ MAX Butyl Cellufine™ MAX Phenyl Cellufine™ MAX Phenyl LS
Matrix Highly Cross-linked Cellulose
Particle size 40～130 μm
Ligand type Butyl Phenyl
BSA adsorption capacity (mg/ml) 9 11 4
BSA elution efficiency (%) 70 40 90
Polyclonal IgG 10% DBC (mg/ml) 17 30 19
Operating pressure < 0.3 MPa
pH stability pH 2 ~ 13
Storage 20 % Ethanol

Model Protein Separation

Protein elution pattern of Cellufine™ HIC resins
Column
6.6 mm ID x 50 mm L
Buffer A
10 mM phosphate buffer (pH 7) + 1.5 M (NH4)2SO4
Buffer B
10 mM phosphate (pH 7)
Proteins
Ribonuclease A, Lysozyme, α-Chymotripsinogen A

Adsorption Capacity of Model Proteins

Comparison data of protein adsorption capacity of Cellufine™ MAX phenyl and Cellufine™ MAX phenyl LS
Column
5 mm ID x 10 cm L
Protein concentration
1 mg/ml
Buffer
50 mM Tris-HCl (pH 8.5)

Dynamic Binding Capacity for Cellufine™ MAX Phenyl vs. Salt Concentration

Comparison data of protein adsorption capacity of Cellufine™ MAX phenyl and Cellufine™ MAX phenyl LS
Column
5 mm I.D. x 5 cm L
Flow velocity
150 cm/hr
Protein concentration
1 mg/ml
Buffer
20 mM Phosphate (pH7.0) + (NH4)2SO4

Dynamic Adsorption Capacity for Cellufine™ MAX Butyl

Cellufine™ MAX Butyl adsorption capacity data
Column
5 mm I.D. x 5 cm L
Flow rate
0.5 ml/ min
Protein concentration
1 mg/ml
Buffer
10 mM Phosphate (pH 7.0) +
2 M (NH4)2SO4 / BSA
1 M (NH4)2SO4 / polyclonal IgG

Pressure vs. Flow Properties

Flow property of Cellufine™ MAX Phenyl and Cellufine™ MAX Butyl
Column
2.2cm I.D. x 20 cm L
Temperature
24 ± 1 ℃
Mobile phase
Water

Chemical and Caustic Stability

  • Ethanol (70%)
  • NaOH (0.5M)
  • Isopropyl alcohol (30%)
  • Detergents
  • Guanidine hydrochloride (6M)
  • Autoclave (121 ºC, 20 min)
  • Urea (6M)
Cellufine™ MAX Butyl
Cellufine™ MAX Phenyl
Cellufine™ MAX Phenyl LS

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