CellufineTM Mini-Column
Effective tool for protein purification
Cellufine Mini-Column is a prepacked column
Cellufine Mini-Columns offer convenience and expedience for isolating, purifying, and concentrating biomolecules from aqueous samples.
Application Data
NEWInfluenza Virus Separation by Cellufine Sulfate Mini-Column
Inactivated influenza H5N1 type from allantoic fluid was purified with Cellufine Sulfate mini-column.
Conditions
- Volume of Cellufine Sulfate
- 1ml （ID 8×20 mm)
- Liner Velocity
- 120cm/hr
- Equilibration buffer
- 0.01M Phosphate buffer, pH 7.4
- Washing buffer
- 0.01M Phosphate buffer, 0.19M NaCl, pH7.2
- Elution buffer
- 0.01M Phosphate buffer, 3M NaCl, pH7.0
- Virus Strain
- A/duck/Hokkaido/Vac-1/04 (H5N1) β-Propiolactone
--- Allantoic Fluid was filtered after centrifuged
(Centrifugation: 8,000g×30min, Filter:0.45μm Cellulose Acetate) - Sample Volume
- 6 ml(The virus sample from professor Kida.(Hokkaido Univ. Graduate school of veterinary medicine. )
Dynamic Binding Capacity (DBC) of lysozyme on Cellufine Sulfate
No significant change in DBC was observed over a flow range of 0.5 to 5 ml/min
Cellufine SPA-HC
| Quantity | Catalogue No. |
|---|---|
| 1ml x 1 | 21900-11 |
| 1ml x 5 | 21900-51 |
| 5ml x 1 | 21900-15 |
Cellufine Sulfate
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 19845-51 |
| 5ml x 1 | 19515 |
Cellufine MAX DexS-HbP
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 21 700-51 |
| 5ml x 1 | 21 700-15 |
Cellufine MAX DexS-VirS
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 21 800-51 |
| 5ml x 1 | 21 800-15 |
Cellufine ET clean S
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20151 |
| 5ml x 1 | 20115 |
Cellufine ET clean L
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20051 |
| 5ml x 1 | 20015 |
Cellufine PB
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20251 |
| 5ml x 1 | 20215 |
Cellufine Phosphate
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 19551 |
| 5ml x 1 | 19515 |
Cellufine Chelate
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 19875-51 |
| 5ml x 1 | 19875-15 |
Cellufine MAX Butyl
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 21100-51 |
| 5ml x 5 | 21100-55 |
Cellufine MAX Phenyl
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20700-51 |
| 5ml x 5 | 20700-55 |
CellufineMAX Phenyl LS
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20800-51 |
| 5ml x 5 | 20800-55 |
Cellufine A-200
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 19611-51 |
Cellufine A-500
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 19805-51 |
| 5ml x 5 | 19805-55 |
Cellufine A-800
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 19865-51 |
| 5ml x 5 | 19865-55 |
Cellufine C-500
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 19800-51 |
| 5ml x 5 | 19800-55 |
Cellufine Q-500
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 19907-51 |
| 5ml x 5 | 19907-55 |
Cellufine S-500
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 21200-51 |
| 5ml x 5 | 21200-55 |
Cellufine MAX S-r
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20300-51 |
| 5ml x 5 | 20300-55 |
Cellufine MAX S-h
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20400-51 |
| 5ml x 5 | 20400-55 |
Cellufine MAX GS
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 21300-51 |
| 5ml x 5 | 21300-55 |
Cellufine MAX Q-r
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20500-51 |
| 5ml x 5 | 20500-55 |
Cellufine MAX Q-h
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20600-51 |
| 5ml x 5 | 20600-55 |
Cellufine MAX CM
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 20900-51 |
| 5ml x 5 | 20900-55 |
Cellufine MAX DEAE
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 21000-51 |
| 5ml x 5 | 21000-55 |
Cellufine MAX IB
| Quantity | Catalogue No. |
|---|---|
| 1ml x 5 | 21600-51 |
| 5ml x 1 | 21600-15 |
Cellufine GH-25
| Quantity | Catalogue No. |
|---|---|
| 5ml x 5 | 19711-55 |
