Gel Filtration
Chromatography Media

CellufineTMGCL-2000
CellufineTMGCL-2000HF

Gel Filtration, or size exclusion chromatography, has proven to be a simple and effective purification method for proteins and other biomolecules. However, the traditionally slow flow rates and limiting resolving power of most gel filtration media have restricted its usefulness. These problems, inherent to the packings, existed not only at the laboratory preparative scale but especially at process scale. The limited throughput of gel filtration columns has often created a significant process bottleneck.
Cellufine GCL media overcome these limitations. The mechanical strength of the spherical cellulose matrix allows high flow rates, even in large industrial columns.

Features

  • Rigid spherical particles
  • Outstanding mechanical strength
  • Hydrophilic
  • High pore volume
  • Broad selection of fractionation range
  • Supplied preswollen
  • High chemical resistance
  • Autoclavable at 121 °C for 30 minutes

Benefit

  • High flow rate compatibility even in large diameter process columns
  • May be pumped or stirred without fines generation
  • Low non-specific adsorption and high recovery
  • Good capacity
  • Optimize selectivity to required molecular weight range
  • No time-consuming swelling procedures required
  • Usable with all common salts, detergents, chaotropic agents, organic solvents
  • Depyrogenation
  • Sterilizable
  • Regulatory Support
Characteristics
Matrix Cross linked cellulose
Particle Shape Spherical
Particle Size ca. 40-130 μm
Chemical Resistance Media can be used with most salts, detergents, solvents, acids and bases, including 8M urea, 6M guanidine / HCL, 0.1M HCL and 0.5M NaOH
Mechanical Resistance Media will withstand peristaltic pumping and extended magnetic stirring
Schrinkage / Swelling Shrinkage or swelling < 3 % with changes in pH or ionic strength. Shrinkage or swelling with solvents varies with solvent and media
Autoclavable 121 °C for 30 minutes
Supplied Suspension in 20 % ethanol

Range of Fractionalization

This media offer an extraordinarily broad selection of fractionation ranges: from the unique Cellufine GCL-2000 and Cellufine GCL-2000HF for very high molecular weight protein complexes. Table 1 shows the exclusion limit for the media.

Protein MW
IgM 900,000
Thyrogloburin 660,000
IgG 155,000
BSA 66,000
Chymotrypsinogen A 25,700
α-Chymotrypsin 25,200
Myoglbin 17,000
Lysozyme 14,300
Rnase 13,700
Cytochrome c 12,400
Bacitracin 1,400
Glycine 75

Table 1

Range of Fractionalization
Figure 1

Pressure Flow Curves

Pressure Flow Curves

Protein Recovery

Protein % Recovery
Catalase 100
lgG 100
Aldolase 100
Serum Albumin 100
Lactoglobulin 100
Ribonuclease 100
Cytochrome C 100
Lysozyme 100
Chymotrypsinogen A 99
Myoglobin 97

Development of the grade

Development of the grade according to demand of a customer (inquiry)

Back to Top