Gel Filtration
Chromatography Media


Size exclusion chromatography is a separation mode that separates proteins by the difference in molecular weight. In principle, it is necessary to reduce the particle size to improve the separation performance. Therefore it causes difficulty of operating high flow rate because of its pressure loss. This feature of size exclusion chromatography is a bottleneck in process design. Cellufine GCL-2000HF was developed to eliminate this bottleneck. Since it is based on spherical cross-linked cellulose particles, it has high physical strength and can be operated at high flow rate, so it can be used on an industrial scale. In addition, it has a pore size optimized for protein separation, so it exhibits excellent separation performance.


  • Rigid spherical particles
  • Outstanding mechanical strength
  • Hydrophilic
  • High pore volume
  • Broad selection of fractionation range
  • Supplied preswollen
  • High chemical resistance
  • Autoclavable at 121 °C for 30 minutes


  • High flow rate compatibility even in large diameter process columns
  • May be pumped or stirred without fines generation
  • Low non-specific adsorption and high recovery
  • Good capacity
  • Optimize selectivity to required molecular weight range
  • No time-consuming swelling procedures required
  • Usable with all common salts, detergents, chaotropic agents, organic solvents
  • Depyrogenation
  • Sterilizable
  • Regulatory Support
Matrix Cross linked cellulose
Particle Shape Spherical
Particle Size ca. 40-130 μm
Chemical Resistance Media can be used with most salts, detergents, solvents, acids and bases, including 8M urea, 6M guanidine / HCL, 0.1M HCL and 0.5M NaOH
Mechanical Resistance Media will withstand peristaltic pumping and extended magnetic stirring
Schrinkage / Swelling Shrinkage or swelling < 3 % with changes in pH or ionic strength. Shrinkage or swelling with solvents varies with solvent and media
Autoclavable 121 °C for 30 minutes
Supplied Suspension in 20 % ethanol

Range of Fractionalization

Cellufine GCL-2000HF can be separating proteins with a wide range of molecular weight. Figure 1 and Table 1 show the results of measuring the exclusion limit of GCL-2000HF.

Protein MW Kav
Thyrogloburin 660,000 0.00
IgG 155,000 0.45
BSA 66,000 0.52
Chymotrypsinogen A 25,700 0.62
α-Chymotrypsin 25,200 0.62
Myoglbin 17,000 0.63
Lysozyme 14,300 0.70
Ribonuclease A 13,700 0.70
Bacitracin 1,400 0.91

Table 1 Relationship between proteins and Kav

Proteins of different molecular weights were loaded into a column packed Cellufine GCL-2000HF. Kav was measured based on the amount of volume eluted from the column.

Kav data of Cellufine GCL-2000HF
Figure 1

Pressure Flow Curves

Flow property of Cellufine GCL-2000HF

Protein Recovery

Protein % Recovery
Catalase 100
lgG 100
Aldolase 100
Serum Albumin 100
Lactoglobulin 100
Ribonuclease 100
Cytochrome C 100
Lysozyme 100
Chymotrypsinogen A 99
Myoglobin 97

Excellent stability (accelerated test)

Cellufine GCL-2000HF is a size exclusion chromatography resin cross-linked with cellulose particles that are extremely stable as a substance.

Accelerated test of Cellufine GCL-2000HF
Figure 3 Stability when stored at 40 ° C

Cellulose is a heat-stable polysaccharide. The agarose, which is commonly used as a chromatography resin, has many hydroxyl groups because it is the same polysaccharide as cellulose. Therefore, like cellulose, it has the characteristic of less non-specific adsorption of proteins. On the other hand, agarose is easily dissolved in hot water. For this reason, agarose has the disadvantage of being sensitive to heat. Although cellulose is a polysaccharide with hydroxyl groups, it has an extremely excellent feature of resistance for heat because of crystalline structure in it.

Excellent stability (preservation test)

Cellufine GCL-2000HF was stored at a temperature of 30 ° C in a 20% ethanol solution, and the subsequent changes in pore size were investigated (Fig. 4). It was found that Cellufine GCL-2000HF shows excellent stability over a long period.

Preservation test of Cellufine GCL-2000HF
Figue 4 Long-term storage stability

Custom Resin Development Services

JNC offers a custom resin development service for optimization of current products to meet stringent or enhanced performance working directly with large and small customers. In addition, JNC also offers custom resin development services to support new applications of JNC resins.

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