Ion Exchange
Chromatography Media

Cellufine™ A, Q, C, and S

For protein peptide and other biomolecule purification

Cellufine Ion Exchangers are based on spherical particles manufactured from crosslinked cellulose. Each offers excellent flow properties, mechanical stability and chemical resistance. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides and other biomolecules. Applications include the purification of antibodies, growth factors, albumin, enzymes, nucleic acids, etc.

Features

  • Spherical particles exhibiting high mechanical strength
  • Choice of several pore sizes
  • Hydrophilic
  • DEAE (A), QA (Q) anion exchange, carboxylmethyl (C) and Sulfobutyl (S)cation exchange
  • Pre-swollen
  • Virtually no shrinkage or swelling
  • Resistant to 0.5M NaOH
  • Stable in organic solvents
  • Autoclavable (121 °C for 30 minutes)
  • Passes USP VI acute systemic implant tests

Benefits

  • High flow rates allowing rapid chromatography and direct scale-up
  • Allows optimum capacity matched to molecular weight
  • Low non-specific binding
  • Optimal functionality selection
  • Easy packing
  • Easy large scale operation
  • Easy regeneration and depyrogenation
  • Allows alternative cleaning regimes
  • Sterilizable
  • Non toxic
  • Regulatory support

Partial Structure

Cellufine A-200,A-500, A-800

Cellufine A-200,A-500, A-800

Cellufine Q-500

Cellufine Q-500

Cellufine C-500

Cellufine C-500

Cellufine S-500

Cellufine S-500
Characteristics
Product Cellufine A-200 Cellufine A-500 Cellufine A-800 Cellufine Q-500 Cellufine C-500 Cellufine S-500
Ion Exchange Type (Functional Group) Weak Anion (DEAE) Strong Anion(QA) Weak Cation (CM) Strong Cation (S)
Base Matrix Spherical, Cross-linked Cellulose Beads
Particle Size (μm) ca. 40-130
Exclusion Limit (kDa) > 30 > 500 > 1,000 > 500 > 500 > 500
pH Working Range 2 – 12 2 - 13
Operating Pressure Up to 2 bar ( 0.2 MPa)
Shrinkage/ Swelling < 5% on change Negligible
Ion Exchange Capacity(meq / ml-gel) 0.13 - 0.18 0.13 - 0.17 0.05 - 0.08 0.14 - 0.29 0.07 - 0.14 0.11 - 0.22
Dynamic Binding Capacity (mg/ml) BSA* or Lysozyme** 46* 57* 84* 16* 130** 156**
human-γ-globulin 38 42 68 10 58 42
Supplied Suspension in 20 % EtOH

Titration Curves

Titration Curves
Figure1

Adsorption Capacity

Adsorption Capacity
Column
5mm ID × 50 mm L
Liner Velocity
150 cm/Hr
BSA concentration
1 mg/ml in 50mM
Tris-HCl (pH8.5)
poly IgG concentration:1mg/ml in 50mM Tris-HCl (pH9.5)
Adsorption Capacity
Adsorption condition
Column
5mm I.D. ×50mm L
Liner Velocity
150 cm/Hr (Strong Cation)
Lysozyme
Tris-HCl (pH8.5)
polyclonal IgG
10mM acetate pH4.3 + 50mM sodium chloride (Weak Cation)
Lysozyme concentration
1mg/ml in 50mM Tris-HCl (pH8.5)
polyclonal IgG concentration
1mg/ml in 10mM acetate (pH5.6)
Adsorption Capacity
Adsorption condition
Column
5mm I.D. × 50mm L
Liner Velocity
150 cm/Hr
BSA concentration
1mg/ml
Buffer
50mMTris-HCl(pH8.5)plus NaCl

Flow Properties

Pressure flow curves for Cellufine Ion Exchangers in laboratory and process scale columns.

Flow Properties
Figure2
Flow Properties
Figure3

Mechanical Resistance

Cellufine Ion Exchangers are semi-rigid due to the crosslinked structure which gives high mechanical resistance and results in two advantages; no generation of fines during the preparation of the column and higher operating bed height without concern of bed collapse common to many ‘soft gels’.

Chemical Resistance

Cellufine is chemically stable. The media can be washed with a variety of solutions and shows no loss in capacity, resolution or flow rates.

Solvent Exposure without loss at room temperature
Methanol Compatible
Ethanol Compatible
6M Guanidine HCI Compatible
8M Urea Compatible
1% Triton X Compatible
1M Acetic Acid 7 days
0.5M NaOH 30 days
0.5M NaOH +1M NaCl 30 days
0.1M HCl 30 days

Applications

Applications
Figure4
Applications
Figure5
Applications
Figure6

Development of the grade

Development of the grade according to demand of a customer (inquiry)

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