Desoxycholate agar medium |
- 1）For 200 ml of purified water
→9g of medium is heated and melted
- 2）Dispense into a petri dish. (Do not sterilize!)
Medium storage is not possible.
|
Selective isolation of coliformsBacterial count calculation
|
35℃ / 24 hour |
BGLB bouillon medium |
- 1）For 200 ml of purified water
→8g of medium is heated and melted
- 2）Put a Durham tube (6 x 30) in a medium test tube (Φ18.0 x 180) and dispense the dissolved BGLB medium. Cover with a Molton stopper, sterilize with steam at 121 ° C for 15 minutes, Then cool medium and then cool rapidly.
|
Coliforms estimation test
Bacterial cell calculation(for nutrient-rich samples)
|
37℃ / 24-48 hour |
EC Bouillon Medium |
- 1） For 200 ml of purified water
→Dissolve 7.4g of medium.
- 2）Prepare in the same way as the above BGLB medium.
|
Detection of E.coli and coliforms (estimated test)
|
37℃ or 45.5℃ / 48 hour |
Lactose bouillon medium (BTB added)
|
- 1） For 200 ml of purified water
→Dissolve 2.6g of medium.
- 2）Prepare in the same way as the above BGLB medium.
|
Coliforms / E. coli estimation test and complete test
|
37℃ / 24-48 hour |
EMB agar medium |
- 1）For 200 ml of purified water
→Dissolve 7.2g of medium.
- 2）Sterilize with high pressure steam at 121 ° C for 15 minutes and dispense into a petri dish.
|
Sorting of pathogenic gut flora
Appraisal separation
|
37℃ / 24 hour |
