Preparation of medium

Medium Preparation method Usage Culture temperature / time

Standard agar medium
  1. 1）For 200 ml of purified water
    → Dissolve 4.5g.
    ＊Add 1.0 g of skim milk per liter of purified water as needed.
  2. 2）Sterilize with high pressure steam at 121 ° C for 15 minutes and flow into a petri dish.。
 Measurement of the number of bacteria in a sample
＊Skim milk should be added when inspecting milk and dairy products.		 32～35℃/48hr

BCP-added plate count agar medium
  1. 1)For 200 ml of purified water
    → Dissolve 4.92g.
  2. 2)Sterilize with high pressure steam at 121 ° C for 15 minutes and pour into a petri dish.
 Measurement of the number of lactic acid bacteria 35℃/48hr