Preparation of medium

Medium Preparation method Usage Culture temperature / time

Peptone saline
  1. 1) For 200 ml of purified water
    →Dissolve 1.9g of medium.
  2. 2) The medium is autoclaved for 15 minutes at 121 ° C and dispensed into test tubes.
 Enriched culture of Vibrio parahaemolyticus
or diluting other food samples		 35℃ / 24 hours

TCBS agar medium
  1. 1) For 200 ml of purified water
    →Dissolve 17.6g of medium.
  2. 2) Dispense into a petri dish (Do not sterilize)
Selective separation of Vibrio parahaemolyticus 37℃ / 18 to 24 hours