Membrane filter inspection method using MC-Media Pad

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Membrane filter inspection method using "MC-Media Pad"
   

Inspection method


1. Features of the membrane filter method


This is an inspection method used when inspecting liquid samples with relatively few microorganisms such as water and beverages. The bacteria are captured with a membrane filter and transferred to the MC-Media Pad to measure the number of colonies.

2. Prepare the following equipment.

MC-Media Padを用いた食品検査法:メンブランフィルター準備

3. Add 1 ml of sterilized water to the pad part of the "MC Media Pad" in advance for the required quantity. (Leave it for at least 15 minutes to let it soak in sterilized water.)



※If necessary, sterilized water can be added the day before, put it in an aluminum bag, and store it in the refrigerator.

4. Set the sterilized membrane filter and filter the sample solution.

MC-Media Padを用いた食品検査法:メンブランの設置とろ過

5. Place the filtered membrane filter on the "MC-Media Pad".

MC-Media Padを用いた食品検査法:メンブランへの転写

6. Place the "MC-Media Pad" in the incubator and incubate.

MC-Media Padを用いた食品検査法:培養

Bacteria               ;35℃ 24 hours to 48 hours
Coliforms             ;35℃ 24 hours
Staphylococcus aureus;35℃ 24 hours


7. Judge.


MC-Media Padを用いた食品検査法:コロニーカウント