1) Prepare the following equipment.

2) Add 1 ml of sterilized water to MC-Media Pad, leave it for at least 15 minutes with colosing the cover film.
※If necessary, sterilized water can be added the day before, store in the refrigerator in an aluminum bag.

3) Set the sterilized membrane filter and filter the sample solution. Record the volume of filtered solution.

4) Put the filtered membrane on the MC-Media Pad, close the cover film.
5) Put this MC-Media Pad into incubator, incubate at the temperature and time specified for each product.
6) Take out the MC-Media Pad from incubator, count the number of colonies with interpretation for each product.
7) Culculate tne number of microorganisms in the sample solution with counted colonies and filtered volume.
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