Membrane filter method with MC-Media Pad


Various inspection methods


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Membrane filter inspection method using "MC-Media Pad"
   

Purpose of inspection


Features of the membrane filter method  This is an inspection method used when inspecting liquid samples with relatively few microorganisms such as water and beverages. The bacteria are captured with a membrane filter and transferred to the MC-Media Pad to measure the number of colonies.


Inspection method


1) Prepare the following equipment.


MC-Media Padを用いた食品検査法:メンブランフィルター準備


2) Add 1 ml of sterilized water to MC-Media Pad, leave it for at least 15 minutes with colosing the cover film.


※If necessary, sterilized water can be added the day before, store in the refrigerator in an aluminum bag.


3) Set the sterilized membrane filter and filter the sample solution. Record the volume of filtered solution.


MC-Media Padを用いた食品検査法:メンブランの設置とろ過


4) Put the filtered membrane on the MC-Media Pad, close the cover film.


MC-Media Padを用いた食品検査法:メンブランへの転写


5) Put this MC-Media Pad into incubator, incubate at the temperature and time specified for each product.


MC-Media Padを用いた食品検査法:培養


6) Take out the MC-Media Pad from incubator, count the number of colonies with interpretation for each product.


MC-Media Padを用いた食品検査法:コロニーカウント


7) Culculate tne number of microorganisms in the sample solution with counted colonies and filtered volume.