Food inspection procedure using MC-Media Pad

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Food inspection procedure using MC-Media Pad
　　　

What is aerobic bacteria?

The aerobic count is regarded as a hygienic indicator that evaluates the hygienic quality of food. The aerobic count is defined as the mesophilic bacterial count that has grown on standard agar medium at 35 ° C for 48 hours under aerobic conditions. Also known as aerobic count or standard tablet cell count. Bacteria include spore-forming bacteria, and once spores are formed, they cannot be killed by heating or sterilizing with alcohol. In this case, it is necessary to create an environment that does not increase bacteria.

Significance of the test: By testing the viable cell count, it is an index of whether or not the environment (moisture, temperature, dirt, etc.) where the bacteria are likely to grow. When the number of viable bacteria is high, it is necessary to improve the environment.

General criteria: Heated foods…10５pieces / g or less,　Unheated foods…106pieces / g or less
Example of general management standard; wiping inspection (after cleaning)…10３pieces / ㎠ or less


Comparison of inspection methods

For general inspection

For inspection using MC-Media Pad
   
   
   
 
 





It is detected as a red settlement.
The indicator turns red due to the respiration of microorganisms.

Inspection method

1) Enter the test date, sample number, and dilution ratio on the color tape part of the "MC-Media Pad" cover film.
MC-Media Padを用いた食品検査方法：測定前準備

2) Make a 10-fold diluted solution.
MC-Media Padを用いた食品検査：検体の希釈方法

3) 110倍希釈液を「MC-Media Pad」に添加します。
MC-Media Padを用いた食品検査法：試料の添加

4) Make a 100-fold diluted solution and add it to "MC-Media Pad".
MC-Media Padを用いた食品検査法：100倍希釈液の添加

5) Make a 1000-fold diluted solution and add it to "MC-Media Pad".
MC-Media Padを用いた食品検査法：1000倍希釈液の添加

　

6) Place the "MC-Media Pad" in the incubator and incubate.
MC-Media Padを用いた食品検査法：培養
Culture time
ACplus；35 ℃ 24-48 hours
EC；35℃24 hours

7) Judge.

Mark the colony with magic and count the number of bacteria.

8) Judgment method

After culturing, count the number of colonies that appear in "MC-Media Pad" Acplus and EC. You can only count sheets in the range (less than 1000 pieces). EC can measure the number of E. coli and the number of coliform bacteria at the same time.
MC-Media Pad ECの発色見本

9) From the result of ⑧, multiply the dilution ratio to obtain the number of bacteria.

　・In the case of 10-fold diluted solution: Number of bacteria = Number of counted bacteria x 10
　・In the case of 10-fold diluted solution: Number of bacteria = Number of counted bacteria x 100
　・In the case of 10-fold diluted solution: Number of bacteria = Number of counted bacteria x 1000

10) Calculate the average of Acplus and EC, and use them as the aerobic count, coliforms count, and E.coli count.
The unit is "cfu / g" or "pieces / g".

MC-Media Padを用いた食品検査法：菌数の測定

※１　In the case of coliforms, the whole of the sheet media may develop from pale bluish green to blue due to the action of the enzyme that raw vegetables have. The color develops in a short time (from about 2 hours) after starting the culture. As a result, it may be difficult to see the colonies that develop color, so please change the above explanation to the following for foods that cause such a phenomenon.

1) Add the 100-fold diluted solution of 4) of the above inspection procedure.



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