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Hydrophobic Interaction Chromatography Media
Cellufine™ MAX Butyl,
Cellufine™ MAX Phenyl

Cellufine™ MAX is a 2nd generation Cellufine media with high flow characteristics. JNC developed a new, highly cross-linked base resin for Cellufine MAX series. Cellufine MAX hydrophobic interaction chromatography is now available with MAX Phenyl and Butyl chemistries.



SEM analysis of Cellufine MAX base resin
Cellulose, a natural polysaccharide, possesses unique crystalline molecular structure differing from non-crystalline polysaccharides such as agarose. Thus Cellufine has distinctive pore structure as shown in the pictograph (Fig. 1). The new Cellufine MAX series offers the largest pore size of all Cellufine chromatography media. The benefit of such pore size in Cellufine MAX HIC media provides superior strength and excellent mass transfer.

Cellufine MAX Butyl > MSDS / Technical Information > Catalog No.


Cellufine MAX Phenyl > MSDS / Technical Information > Catalog No.


Cellufine MAX Phenyl LS > MSDS / Technical Information > Catalog No.



Partial structure
Hydrophobic Interaction Cellufine MAX Butyl

Cellufine MAX Phenyl
Cellufine MAX Phenyl LS



Characteristics
Characteristics
Type Cellufine MAX Butyl Cellufine MAX Phenyl Cellufine MAX Phenyl LS
Matrix Highly Cross-linked Cellulose
Particle size 40~130 μm
Ligand type Butyl Phenyl
BSA adsorption capacity (mg/ml) ≧ 9 ≧ 11 ≧ 4
BSA elution efficiency (%) > 70 > 35 > 65
Polyclonal IgG 10% DBC (mg/ml) 17 30 19
Operating pressure < 0.3 MPa
pH stability pH 2 ~ 13
Storage 20 % Ethanol

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Model Protein Separation
 
Column: 6.6 mm ID x 50 mm L
Buffer A: 10 mM phosphate buffer (pH 7) + 1.5 M (NH4)2SO4
Buffer B: 10 mM phosphate (pH 7)
Proteins: Ribonuclease A, Lysozyme,
α-Chymotripsinogen A

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Adsorption Capacity of Model Proteins
 
Column: 5 mm ID x 10 cm L
BSA concentration: 1 mg/ml
Buffer: 50 mM Tris-HCl (pH 8.5)

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Dynamic Binding Capacity for Cellufine MAX Phenyl vs. Salt Concentration
Column: 5 mm I.D. x 5 cm L
Flow velocity:150 cm/hr
BSA concentration: 1 mg/ml
Buffer: 20 mM Phosphate (pH7.0) + (NH4)2SO4

Dynamic Adsorption Capacity for Cellufine MAX Butyl
 
Column: 5 mm I.D. x 5 cm L
Flow rate: 0.5 ml/ min
Protein concentration: 1 mg/ml
Buffer: 10 mM Phosphate (pH 7.0) +
2 M (NH4)2SO4 / BSA
1 M(NH4)2SO4 / polyclonal IgG

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Pressure vs. Flow Properties
 
Column:2.2cm I.D. x 20cmL
Temperature: 24±1℃
Mobile phase: Water




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Ligand density is controllable in Cellufine MAX HIC Ex.) Purification of HBsAg by Cellufine MAX Butyl
As ligand density is controllable, Cellufine MAX HIC is optimized for customer’s purpose.
The figure shows a chromatogram of adsorption-desorption of r Hepatitis B virus particles to Cellufine MAX Butyl LS (optimization for HBV) and Agarose typed butyl media

 
  Cellufine MAX Butyl LS Agarose typed Butyl media
Load 163 (100) 163 (100)
Flowthrough 48 (29) 50 (31)
Eluted target 78 (48) 63 (39)
μg(%)

Run condition
HIC resin: Cellufine MAX Butyl LS and Agarose typed butyl media
System: AKTA explorer 10S
Column: Φ3.0 x 50 mm (vol. 0.35 ml)
Sample: 150 mg of r-HBsAg (Beacle Inc.) in 0.02 M PB, 0.6M (NH4)2SO4, pH 7.0
Equilibration and wash: 0.02 M PB, 0.6M (NH4)2SO4, pH 7.0
Elution 1: 0.02 M PB, pH 7.0
Flow rate: 0.25 ml/min(220 cm/hr)

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Chemical and Caustic Stability
Ethanol (70%)   NaOH (0.5M)
Isopropyl alcohol (30%)   Detergents
Guanidine hydrochloride (6M)   Autoclave (121 ºC, 20 min)
Urea (6M)      


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