Hydrophobic Interaction Chromatography Media
Cellufine MAX Phenyl

Cellufine MAX is a 2nd generation Cellufine media with high flow characteristics. JNC developed a new, highly cross-linked base resin for Cellufine MAX series. Cellufine MAX hydrophobic (Phenyl)chromatography is now available in 2 types, which differ in functional ligand density; a standard type and low ligand density type LS.



SEM analysis of Cellufine MAX base resin
Cellulose, natural polysaccharides, possesses unique crystalline molecular structure differing from non-crystalline polysaccharides such as agarose. Thus Cellufine has unique pore structure. The new Cellufine MAX series offers the largest pore size of all Cellufine chromatography media. The benefit of such pore size in Cellufine media provides superior strength and excellent mass transfer.


Partial structure
Hydrophobic (Phenyl)
Cellufine MAX Phenyl
Cellufine MAX Phenyl LS


Characteristic
Characteristics
Type Cellufine MAX Phenyl Cellufine MAX Phenyl LS
Matrix Highly cross-linked cellulose
Particle size ca. 40~130 μm
Ligand type Phenyl
BSA adsorption capacity (mg/ml) 14 6
Polyclonal IgG 10% DBC (mg/ml) 31 19
Operating pressure < 0.3 MPa
pH stability pH 2〜13
Storage 20 % ethanol

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Model Protein Separation
 
Column: 6.6 mm Diam. x 5 cm L
Protein: Ribonuclease A,Lysozyme,
α-Chymotripsinogen A
Elution: 10m M PB (pH7.0)1.5 → 0 M (NH4)2SO4 gradient

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Adsorption Capacity of Model Proteins
 
Column: 5 mm ID x 10 cm L
BSA concentration: 1 mg/ml
Buffer: 50 mM Tris-HCl (pH 8.5)

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Dynamic Binding Capacity vs. Salt Concentration
 
Column: 5 mm Diam. x 5 cm L
Flow velocity: 150 cm/hr
Protein concentration: 1 mg/ml
Buffer: 20mM Phosphate + (NH4)2SO4

 
Column: 5 mm Diam. x 5 cm L
Flow velocity: 150 cm/hr
Protein concentration: 1 mg/ml
Buffer: 20mM Phosphate + (NH4)2SO4

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Customization (Control of Ligand Density)
JNC offers optimized Cellufine MAX Phenyl on customer’s request

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Pressure vs. Flow Properties
 
Column: 2.2 cm Diam. X 20 cm L
Temperature: 24±1 ºC
Mobile phase: water

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Chemical and Caustic Stability
Ethanol (70%)   NaOH (0.5M)
Isopropyl alcohol (30%)   Detergents
Guanidine hydrochloride (6M)   Autoclave (121 ºC, 20 min)
Urea (6M)      

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Ordering Information (pack Size and Catalogue number)
Cellufine MAX Phenyl
Pack size Catalogue No.
1ml x 5 (Mini-Column) 20700-51
5ml x 5 (Mini-Column) 20700-55
100ml 20700
500ml 20701
5 lt 20702
10 lt 20703
pdf MSDS
pdf Operating Instructions
pdf Technical data sheet
Cellufine MAX Phenyl LS
Pack size Catalogue No.
1ml x 5 (Mini-Column) 20800-51
5ml x 5 (Mini-Column) 20800-55
100ml 20800
500ml 20801
5 lt 20802
10 lt 20803


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