JNC CORPORATION : Cellufine : Products

Ion Exchange Chromatography Media
Cellufine™ A, Q, C, and S

For protein peptide and other biomolecule purification

Cellufine Ion Exchangers are based on spherical particles manufactured from crosslinked cellulose. Each offers excellent flow properties, mechanical stability and chemical resistance. These ion exchangers are ideally suited for both laboratory and process scale chromatography of proteins, peptides and other biomolecules. Applications include the purification of antibodies, growth factors, albumin, enzymes, nucleic acids, etc.

Cellufine A-200 > Reference > MSDS / Technical Information > Catalog No.


Cellufine A-500 > Reference > MSDS / Technical Information > Catalog No.

Cellufine A-800 > Reference > MSDS / Technical Information > Catalog No.

Cellufine C-500 > Reference > MSDS / Technical Information > Catalog No.

Cellufine Q-500 > Reference > MSDS / Technical Information > Catalog No.

Cellufine S-500 > Reference > MSDS / Technical Information > Catalog No.


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Features

 

► Spherical particles exhibiting high mechanical strength

► Choice of several pore sizes

► Hydrophilic

► DEAE (A), QA (Q) anion exchange, carboxylmethyl (C) and Sulfobutyl (S)cation exchange

► Pre-swollen

► Virtually no shrinkage or swelling

► Resistant to 0.5M NaOH

► Stable in organic solvents

► Autoclavable (121 °C for 30 minutes)

► Passes USP VI acute systemic implant tests



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Benefits

 

► High flow rates allowing rapid chromatography and direct scale-up

► Allows optimum capacity matched to molecular weight

► Low non-specific binding

► Optimal functionality selection

► Easy packing

► Easy large scale operation

► Easy regeneration and depyrogenation

► Allows alternative cleaning regimes

► Sterilizable

► Non toxic

► Regulatory support



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Partial Structure

Cellufine A-200,
A-500, A-800
Cellufine Q-500
Cellufine C-500
Cellufine S-500

Characteristics
Product Cellufine
A-200
Cellufine
A-500
Cellufine
A-800
Cellufine
Q-500
Cellufine
C-500
Cellufine
S-500
Ion Exchange Type (Functional Group) Weak Anion (DEAE) Strong Anion(QA) Weak Cation (CM) Strong Cation (S)
Base Matrix Spherical, Cross-linked Cellulose Beads
Particle Size (μm) ca. 40-130
Exclusion Limit (kDa) > 30 > 500 > 1,000 > 500 > 500 > 500
pH Working Range 2 – 12 2 - 13
Operating Pressure Up to 2 bar ( 0.2 MPa)
Shrinkage/ Swelling < 5% on change Negligible
Ion Exchange Capacity
(meq / ml-gel)
0.13 - 0.18 0.13 - 0.17 0.05 - 0.08 0.14 - 0.29 0.07 - 0.14 0.11 - 0.22
Dynamic Binding Capacity (mg/ml) BSA* or Lysozyme** 46* 57* 84* 16* 130** 156**
human-γ-globulin 38 42 68 10 58 42
Supplied Suspension in 20 % EtOH


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Titration Curves

Figure1


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Adsorption Capacity



 
Column: 5mm ID ×
50 mm L
Liner Velocity :150 cm/Hr
BSA concentration :1 mg/ml in 50mM
Tris-HCl (pH8.5)
poly IgG concentration:1mg/ml in 50mM Tris-HCl (pH9.5)



Adsorption condition :
Column: 5mm I.D. ×50mm L
Liner Velocity :150 cm/Hr (Strong Cation)
Lysozyme : Tris-HCl (pH8.5)
polyclonal IgG :10mM acetate pH4.3 + 50mM sodium chloride (Weak Cation)
Lysozyme concentration :1mg/ml in 50mM Tris-HCl (pH8.5)
polyclonal IgG concentration:1mg/ml in 10mM acetate (pH5.6)

 
Adsorption condition :
Column: 5mm I.D. ×
50mm L
Liner Velocity :150 cm/Hr
BSA concentration:1mg/ml
Buffer:50mMTris-HCl(pH8.5)plus NaCl


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Flow Properties


Pressure flow curves for Cellufine Ion Exchangers in laboratory and process scale columns. 


Figure2   Figure3


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Mechanical Resistance

 

Cellufine Ion Exchangers are semi-rigid due to the crosslinked structure which gives high mechanical resistance and results in two advantages; no generation of fines during the preparation of the column and higher operating bed height without concern of bed collapse common to many ‘soft gels’. 



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Chemical Resistance

 

Cellufine is chemically stable. The media can be washed with a variety of solutions and shows no loss in capacity, resolution or flow rates. 


Solvent Exposure without loss at room temperature
Methanol Compatible
Ethanol Compatible
6M Guanidine HCI Compatible
8M Urea Compatible
1% Triton X Compatible
1M Acetic Acid 7 days
0.5M NaOH 30 days
0.5M NaOH
+1M NaCl
30 days
0.1M HCl 30 days


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Applications

Figure4
Figure5
Figure6


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