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Affinity Chromatography Media
Cellufine™ Phosphate

For nucleic acid relate protein, dehydrogenase, phosphate relate protein, cation exchange chromatography

Cellufine Phosphate is an affinity media designed for concentration, purification of proteins and, enzymes such as nucleic acid related proteins. Base of media is spherical and rigid cellulose functionalized with Phosphate esters.

Cellufine Phosphate > Reference > MSDS / Technical Information > Catalog No.


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Characteristics
Support Matrix Cellulose
Ligand Phosphate ester
Ligand conc. 0.3 - 0.8meq/ml
Adsorption Capacity ≧ 20mg/ml-gel (lysozyme)

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C.I.P. Stability test


Cellufine Phosphate is stable to cleaning in place (C.I.P) by alkali.


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Chromatography
Column: 1.6x10cm (20ml) packed with Cellufine Phosphate
Flow rate: 3ml/min( 90cm/h )
Sample: 7.5mg of RusA D70N obtained after Heparin-Sepharose chromatography
Gradient: 200ml from 0.1 to 1.3M NaCl in 50mM tris-HCl pH 8.0

SDS-PAGE
Gel: Novex 4-12%BT gel used with MES-SDS running buffer (Invitrogen)
Key for gel: 1 Cell free extract
  2 Unbound material from Heparin-Sepharose column
  3 RusA sample obtained from Heparin-Sepharose column
  4-6 Fractions across the peak eluted from Cellufine Phosphate
  7 RusA reference sample
  8 Mark 12 MW standard (Invitrogen)

Ref.
Nucleic Acids Research, 2006, Vol. 00, No. 00 1–8
Rachel Macmaster, Svetlana Sedelnikova, Patrick J. Baker, Edward L. Bolt1,Robert G. Lloyd1 and John B. Rafferty
RusA Holliday junction resolvase: DNA complexstructure—insights into selectivity and specificity

This data was carried by courtesy of Dr. Svetlana Sedelnikovaof the Sheffield university.

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Column Size: ID 1.1 cm – Height 10 cm
Flow rate: 2 ml/min (126cm/h)
Buffer: 0.01M acetate buffer, pH4.8
Elution: 0 to 1 mol/L NaCl gradient


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